Efficient in vitro plant regeneration from immature embryos of endemic Iris sari and I. schachtii

Authors : Satı UZUN, Ali İrfan İLBAŞ, Arif İPEK, Neşet ARSLAN, Surendra BARPETE

Pages : 348-353

Doi:10.3906/sag-1205-17

View : 20 | Download : 7

Publication Date : 2014-06-01

Article Type : Research Paper

Abstract :Plant tissue culture is an efficient technique for conserving endemic plant species. A reproducible in vitro regeneration protocol was developed for the endemic Iris sari and Iris schachtii in the present study. The highest number of shoots per explant was obtained on Murashige and Skoog insert ignore into journalissuearticles values(MS); medium supplemented with 0.5 mg/L thidiazuron insert ignore into journalissuearticles values(TDZ); plus 0.5 mg/L α-naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); and 1 mg/L TDZ plus 0.5 mg/L NAA, whereby 96.88% and 100% shoot induction with 9.55 and 11.34 shoots per explant of Iris sari and Iris schachtii were recorded, respectively. Regenerated shoots were successfully rooted on MS medium with either 1 mg/L indole-3-butyric acid insert ignore into journalissuearticles values(IBA); or 1 mg/L IBA plus 0.2 mg/L NAA. Rooted shoots were transferred to pots containing either a peat-soil-sand insert ignore into journalissuearticles values(1:1:1); mixture or a hydroponic culture containing Hoagland solution to acclimatize the regenerated plants to the greenhouse chamber. Approximately 90% of plants were transferred ex vitro successfully.
Keywords : Key words Endemic species, immature embryo, in vitro regeneration, Iris sari, Iris schachtii