Phenological changes in the chemical content of wild and greenhouse-grown Hypericum pruinatum: flavonoids

Authors : Cüneyt ÇIRAK, Jolita RADUSIENE, Liudas IVANAUSKAS, Valdas JAKSTAS, Necdet ÇAMAŞ

Pages : 362-370

Doi:10.3906/sag-1305-43

View : 21 | Download : 7

Publication Date : 2014-06-01

Article Type : Research Paper

Abstract :The present study was conducted to determine the phenological changes in the content of main flavonoids, namely amentoflavone, hyperoside, isoquercitrin, quercitrin, quercetin, avicularin, rutin, insert ignore into journalissuearticles values(+);-catechin, and insert ignore into journalissuearticles values(-);-epicatechin in different tissues of Hypericum pruinatum, a promising medicinal herb of the Turkish flora. The wild-growing and greenhouse-grown plants were harvested at vegetative, floral budding, full flowering, fresh fruiting, and mature fruiting stages and dissected into stem, leaf, and reproductive tissues, which were dried separately and subsequently assayed for flavonoid contents by high performance liquid chromatography insert ignore into journalissuearticles values(HPLC);. Chemical contents in whole plants increased during plant phenology and were similar quantitatively for both wild and greenhouse-grown plants. Depending on development stages, reproductive parts produced higher amounts of rutin, quercetin, insert ignore into journalissuearticles values(+);-catechin, insert ignore into journalissuearticles values(-);-epicatectin, and amentoflavone; however, leaves accumulated the highest level of hyperoside, isoquercitrin, quercitrin, and avicularin. The present results indicated a close relationship between flavonoid content in plant parts and phenological development of plants. It is suggested that the raw plant should be harvested during flowering for medicinal purposes.
Keywords : Key words Phytochemical variation, HPLC, Hypericum pruinatum, flavonoids, plant phenology