In vitro screening of octoploid Fragaria chiloensis and Fragaria virginiana genotypes against iron deficiency

Authors : Ayfer ALKAN TORUN, Yıldız AKA KAÇAR, Belgin BİÇEN, Nazife ERDEM, Sedat SERÇE

Pages : 169-179

Doi:10.3906/sag-1303-89

View : 21 | Download : 10

Publication Date : 2014-04-01

Article Type : Research Paper

Abstract :Iron insert ignore into journalissuearticles values(Fe); deficiency is one of the common problems in strawberry insert ignore into journalissuearticles values(Fragaria × ananassa Duch.); growing regions. In vitro screening enables researchers to evaluate genotype performance under several concentrations of Fe in a relatively fast and economical way. Therefore, we evaluated the responses of several genotypes belonging to octoploid Fragaria chiloensis insert ignore into journalissuearticles values(L.); Mill. and Fragaria virginiana Mill. insert ignore into journalissuearticles values(the progenitors of cultivated strawberry); against Fe treatments under in vitro conditions. In the first experiment, HM 1, Pigeon Point, CFRA1267 insert ignore into journalissuearticles values(F. chiloensis subsp. pacifica);, and CA1541 insert ignore into journalissuearticles values(F. chiloensis subsp. chiloensis); were tested with various Fe concentrations insert ignore into journalissuearticles values(0%, 10%, 15%, 25%, 50%, 65%, 80%, and 100%, where 100% = 27.8 mg L-1 FeSO4.7H2O);. Different Fe concentration treatments resulted in differences in plant height, fresh and dry weight, as well as macro- and microelement concentrations among the tested genotypes. In the second experiment, 16 octoploid Fragaria genotypes were tested with 4 Fe concentration treatments insert ignore into journalissuearticles values(0%, 10%, 50%, and 100%, where 100% = 27.8 mg L-1 FeSO4.7H2O);. Iron concentration and genotype interactions were significant for all studied characteristics except sulfur insert ignore into journalissuearticles values(S); and manganese insert ignore into journalissuearticles values(Mn); concentrations, and the 16 genotypes responded differentially to varying Fe treatments. The results of these experiments indicated that F. chiloensis and F. virginiana genotypes exhibited considerable variation under different Fe treatments in vitro, and the more resistant genotypes could be utilized to develop new strawberry cultivars with tolerance to low Fe concentrations.
Keywords : Key words Breeding, genetic resources, iron, strawberry, supercore, tissue culture