Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies

Authors : Tayyaba SHAHEEN, Yusuf ZAFAR, James M. STEWART, Mehboob-ur RAHMAN

Pages : 288-299

Doi:10.3906/tar-1202-35

View : 13 | Download : 8

Publication Date : 2013-06-01

Article Type : Research Paper

Abstract :Microsatellite regions in DNA fragments of Gossypium arboreum were explored and PCR products 500-800 bp long were analyzed for genomic simple sequence repeats insert ignore into journalissuearticles values(gSSRs);. From 39 segments, 23 short gSSRs were identified; on average they occurred every 1.6 kb. Primers were used to amplify fragments from 18 diploid species representing 6 diploid genomes and 1 tetraploid species, G. hirsutum insert ignore into journalissuearticles values(AD);. The phylogeny of diploid genomes was subsequently determined. No primer amplified the DNA from all genotypes; however, amplification occurred in 59% of 437 PCR events. All genomes were grouped into 2 clusters insert ignore into journalissuearticles values(a and b);. The obtained phylogeny of the species parallels previous reports. The strategy adopted here is an efficient method of identifying new gSSRs from species not extensively explored.
Keywords : Key words Gossypium, phylogenetic assessment, RAPD, short gSSRs