Effects of microbial inoculants Enterococcus faecium EF2/3s and EF26/42 on microbial, chemical, and fermentation parameters in grass silage

Authors : Zora VARADYOVA, Dusan JALC, Andrea LAUKOVA, Katarina MIHALIKOVA, Petr HOMOLKA

Pages : 344-351

Doi:10.3906/sag-1208-95

View : 10 | Download : 7

Publication Date : 2013-06-01

Article Type : Research Paper

Abstract :Survival of inoculants in grass silages may enable them to improve the quality of silages through enrichment with polyunsaturated fatty acids insert ignore into journalissuearticles values(PUFAs);. The effects of 2 inoculants, Enterococcus faecium 2/3s insert ignore into journalissuearticles values(EF2/3s); and E. faecium 26/42 insert ignore into journalissuearticles values(EF26/42);, on nutrient composition, fermentation parameters, and fatty acid content in grass silages during ensiling insert ignore into journalissuearticles values(111 days); of fresh grass insert ignore into journalissuearticles values(G); were examined under laboratory conditions. The G [285 g of dry matter insert ignore into journalissuearticles values(DM); kg-1] was ensiled in 36 polyethylene jars insert ignore into journalissuearticles values(1 L); divided into 3 × 12 sets per treatment and ensiled at 21 °C for 111 days. The 3 silage treatments used were: 1); grass without inoculant insert ignore into journalissuearticles values(GS, control);, 2); grass inoculated by the strain EF2/3s insert ignore into journalissuearticles values(GS+EF2/3s);, and 3); grass inoculated by the strain EF26/42 insert ignore into journalissuearticles values(GS+EF26/42);. The inoculant strains were sufficiently established during ensiling and reached 4.62 log10 cfu g-1 for EF2/3s and 3.76 log10 cfu g-1 for EF26/42 on day 111 of ensiling. Crude protein contents were G > GS+EF2/3s > GS+EF26/42 > GS insert ignore into journalissuearticles values(129, 110, 109, and 100 g kg-1 of DM, respectively);. The lactate-to-acetate ratios were GS < GS+EF26/42 < GS+EF2/3s insert ignore into journalissuearticles values(2.82, 4.20, and 4.70, respectively);. Concentrations of a-linolenic acid and g-linolenic acid were highest in grass before ensiling insert ignore into journalissuearticles values(P < 0.001);. Higher isomer C18:2 insert ignore into journalissuearticles values(9,11); content insert ignore into journalissuearticles values(P < 0.01); was detected in GS+EF2/3s and GS+EF26/42 than in GS. Nutritional manipulation associated with Enterococcus faecium EF2/3s and EF26/42 inoculation of GS resulted in better quality of silages based on lower lactate insert ignore into journalissuearticles values(GS+EF26/42); and a greater lactic-to-acetic acid ratio insert ignore into journalissuearticles values(GS+EF2/3s and GS+EF26/42);. This might positively affect PUFAs and their isomers.
Keywords : Key words Degradation, fatty acids, fermentation biotechnology, grass silage, lactic acid bacteria