Micropropagation of Vaccinium arctostaphylos L. via lateral-bud culture

Authors : Mustafa CÜCE, Ersan BEKTAŞ, Atalay SÖKMEN

Pages : 40-44

Doi:10.3906/tar-1204-31

View : 14 | Download : 7

Publication Date : 2013-02-01

Article Type : Research Paper

Abstract :The aim of this study was to determine the most suitable growth medium for rapid and efficient micropropagation of Vaccinium arctostaphylos L. insert ignore into journalissuearticles values(Ericaceae); via lateral-bud cultures. According to preliminary studies, the best explant collection time was late April and May. Accordingly, cuttings were taken from naturally growing V. arctostaphylos populations, and lateral shoot buds were used as explant sources. Anderson`s rhododendron medium insert ignore into journalissuearticles values(AN);, McCown`s woody plant medium insert ignore into journalissuearticles values(WPM);, and Murashige and Skoog insert ignore into journalissuearticles values(MS); basal medium, each supplemented with zeatin/indole-3-butyric acid insert ignore into journalissuearticles values(IBA); insert ignore into journalissuearticles values(1.0/0.1 mg L-1);, were tested to determine the best basal medium for multiple shoot formation; WPM was the most effective basal medium for this purpose. Subsequent shoot multiplication and development studies were carried out with WPM supplemented with an auxin, IBA insert ignore into journalissuearticles values(0.1 mg L-1);, and 2 different cytokinins, zeatin and thidiazuron insert ignore into journalissuearticles values(TDZ);, in various concentrations insert ignore into journalissuearticles values(0.5, 1.0, and 2.0 mg L-1, respectively);. After a 6-week photoperiod insert ignore into journalissuearticles values(16/8 h, light/ dark); application, zeatin/IBA combinations were found to be the most suitable for shoot multiplication and growth. The highest shoot length insert ignore into journalissuearticles values(106.53%); was obtained from the medium supplemented with 1.0/0.1 mg L-1 zeatin/IBA, while the highest increases in leaf number insert ignore into journalissuearticles values(141.89%); and multiple shoot proliferation insert ignore into journalissuearticles values(10.55-fold); were obtained from medium supplemented with 2.0/0.1 mg L-1 zeatin/IBA. WPM was also employed as a rooting medium supplemented with different concentrations of IBA insert ignore into journalissuearticles values(from 0.5 mg L-1 to 8.0 mg L-1); and a 16/8 photoperiod or a completely dark regime. At this stage, the medium supplemented with 0.5 mg L-1 IBA was the best rooting growth regulator with 100% rooting success insert ignore into journalissuearticles values(16/8 photoperiod);. Rooted plantlets transplanted into peat and perlite insert ignore into journalissuearticles values(2:1); substrate were subsequently acclimatized under climate chamber conditions.
Keywords : Key words Indole 3 butyric acid, shoot bud culture, thidiazuron, Vaccinium arctostaphylos, whortleberry, zeatin