Extraction of phenolic compounds from melissa using microwave and ultrasound

Authors : Alev Emine İNCE, Serpil ŞAHİN, Servet Gülüm ŞÜMNÜ

Pages : 69-75

Doi:10.3906/tar-1201-1

View : 14 | Download : 9

Publication Date : 2013-02-01

Article Type : Research Paper

Abstract :In this study, extraction of phenolic compounds from melissa by microwave and ultrasound was studied. In both the microwave and ultrasound extractions, the effects of extraction time insert ignore into journalissuearticles values(5-20 min for microwave; 5-30 min for ultrasound); and solid-to-solvent ratio insert ignore into journalissuearticles values(1:10, 1:20, and 1:30 g mL-1); on total phenolic content insert ignore into journalissuearticles values(TPC); were investigated. Effects of different powers insert ignore into journalissuearticles values(50% and 80%); were also studied for the ultrasound extraction. As a solvent, water was used. In microwave extractions, the highest TPC of extracts insert ignore into journalissuearticles values(145.8 mg GAE g-1 dry material); was obtained in 5 min and at a 1:30 solid-to-solvent ratio. For ultrasound extraction, the conditions that gave the highest TPC insert ignore into journalissuearticles values(105.5 mg GAE g-1 dry material); were 20 min with a 1:30 solid-to-solvent ratio at 50% power. Extracts obtained at the optimum conditions of microwave and ultrasound were compared with conventional extraction and maceration, respectively. TPC and antioxidant activity of the extract was the highest in microwave extraction among all extraction methods. In addition, microwave reduced extraction time by 83%.
Keywords : Key words Leaching, melissa, microwave, phenolic, ultrasound