Incidence and genetic stability of Potato spindle tuber pospiviroid in potato in Turkey

Authors : Üftade GÜNER, Hikmet Murat SİPAHİOĞLU, Mustafa USTA

Pages : 353-363

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Publication Date : 2012-06-01

Article Type : Research Paper

Abstract :The prevalence of Potato spindle tuber pospiviroid insert ignore into journalissuearticles values(PSTVd); infection in randomly selected potato tubers was determined via nonisotopic dot blot hybridization and reverse transcriptase polymerase chain reaction insert ignore into journalissuearticles values(RT-PCR);. In January and February of 2010, 168 seedling tubers of Solanum tuberosum, representing 27 cultivars, were received from different seedling potato producers in Turkey. Digoxigenin-labeled RNA probes of PSTVd were synthesized from the cloned PCR products and applied in dot blot hybridization to detect viroids in RNA extracts isolated from dormant potato tubers. PSTVd was found in 7 samples and 6 cultivars insert ignore into journalissuearticles values(Innovator, Lady Jo, Russet Burbank, Agria, Provento and Konsül);. Some RNA samples, although positive by RT-PCR test, did not give a hybridization signal by dot blot assay. The complete genome of a selected isolate insert ignore into journalissuearticles values(PSTVd-TR); was cloned and sequenced. Primary and secondary structure analysis showed that the RNA genome of the PSTVd-TR isolate insert ignore into journalissuearticles values(GenBank Accession No. HQ456944); differed from other compared isolates by only a few nucleotides. The highest identity insert ignore into journalissuearticles values(99%); was in a PSTVd isolate that was identified in Solanum spp. in Italy insert ignore into journalissuearticles values(Accession No. EF459700);. The infectivity of PSTVd-TR isolate was shown 2 weeks after the mechanical inoculation of sap extracts onto seedlings of tomato cultivar Joker. The PSTVd infections were proven by RT-PCR assay in symptomless tomato seedlings.
Keywords : Key words Dot blot hybridization, molecular characterization, potato, PSTVd