Regeneration and histological analysis of snake melon (Cucumis melo var. flexuosus (L.) Naudin) by direct organogenesis

Authors : Yeşim YALÇIN MENDİ, Selay ELDOĞAN, Rafael GUTAKEV

Pages : 309-317

View : 17 | Download : 8

Publication Date : 2010-08-01

Article Type : Research Paper

Abstract :In vitro morphogenesis of Cucumis melo var. flexuosus insert ignore into journalissuearticles values(L.); Naudin was studied by direct organogenesis. Induction of adventitious buds was obtained from distal and proximal parts of the cotyledon incubated on Murashige and Skoog insert ignore into journalissuearticles values(1962); medium containing different concentrations of 6-benzyladenine insert ignore into journalissuearticles values(BA); insert ignore into journalissuearticles values(0.0, 0.5, 1.0, and 2.0 mg L-1); and indole-3-acetic acid insert ignore into journalissuearticles values(IAA); insert ignore into journalissuearticles values(0.0, 0.1, and 0.5 mg L-1);. The best organogenesis was obtained from the proximal part of the cotyledon on medium containing 0.5 mg L-1 BA and 0.5 mg L-1 IAA insert ignore into journalissuearticles values(88%);. This concentration was used for histological analysis. Less regeneration was obtained on media containing 1.0 mg L-1 BA insert ignore into journalissuearticles values(75%); or 1.0 mg L-1 BA and 0.1 mg L-1 IAA insert ignore into journalissuearticles values(60%);. Plant recovery was much greater from proximal rather than distal explants. A callus formed on almost every explant on media supplemented with BA. Histological analyses showed that the formation of early shoot apical meristems was observed in 14-day-old tissues and meristematic structures were seen in 17-day-old tissues. The first shoot was formed after 21 days. By this time, the surface was covered with leaf primordia and leaves, mostly without accompanying buds.
Keywords : Key words Cell division, cucurbits, in vitro, regeneration, tissue culture