A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities

Authors : Ali SEVİM, Zihni DEMİRBAĞ, İsmail DEMİR

Pages : 333-342

View : 15 | Download : 9

Publication Date : 2010-08-01

Article Type : Research Paper

Abstract :Agrotis segetum Schiff. insert ignore into journalissuearticles values(Lepidoptera: Noctuidae); is one of the most serious pests of nearly all vegetables in Turkey. In this study, to find a more effective and safe biological control agent, the bacterial flora of Agrotis segetum were investigated in larvae collected from different populations in the vegetable fields of the Black Sea region of Turkey, and 9 bacterial isolates were cultured from these populations. Seven of these bacteria were determined and characterized at the species level and the rest were characterized at the genus level. Morphological, physiological, and biochemical characteristics of the bacterial isolates were determined by conventional and routine techniques. Characterization of the isolates was also performed using the VITEK 32 bacterial identification system. In addition, a 16S rRNA gene sequence was determined for these isolates to query against the NCBI genetic database and to construct a phylogenetic tree using closely related species. Consequently, the isolates were identified as Bacillus cereus insert ignore into journalissuearticles values(Ags1);, Bacillus sp. insert ignore into journalissuearticles values(Ags2);, Bacillus megaterium insert ignore into journalissuearticles values(Ags3);, Enterobacter aerogenes insert ignore into journalissuearticles values(Ags4);, Acinetobacter calcoaceticus insert ignore into journalissuearticles values(Ags5);, Enterobacter sp. insert ignore into journalissuearticles values(Ags6);, Pseudomonas putida insert ignore into journalissuearticles values(Ags7);, Enterococcus gallinarum insert ignore into journalissuearticles values(Ags8);, and Stenotrophomonas maltophilia insert ignore into journalissuearticles values(Ags9);. All isolates were determined the first time from the flora of A. segetum. Furthermore, the insecticidal activities of the bacterial isolates were tested against A. segetum larvae, and Enterococcus gallinarum insert ignore into journalissuearticles values(Ags8); was found to cause the highest insecticidal activity insert ignore into journalissuearticles values(60%); 8 days after application.
Keywords : Key words Agrotis segetum, bacterial flora, insecticidal activity