Effects of Stratification, Temperature and Storage on Germination in Three Provenances of Fraxinus angustifolia subsp. oxycarpa Seeds

Authors : Fahrettin TİLKİ

Pages : 323-330

View : 13 | Download : 7

Publication Date : 2005-08-01

Article Type : Research Paper

Abstract :The effects of stratification, temperature and storage on seed germination performance were investigated in 3 provenances of Fraxinus angustifolia subsp. oxycarpa seeds. In the first study, seeds were subjected to 9 different periods of cold stratification or warm stratification followed by cold stratification to overcome dormancy. Stratification and provenances affected seed germination percentage insert ignore into journalissuearticles values(GP); and germination value insert ignore into journalissuearticles values(GV); when seeds were germinated in darkness at 30/20 ºC. Sixteen weeks` cold stratification insert ignore into journalissuearticles values(GP = 40.0, GV = 3.8);, 4 weeks` warm stratification + 4 weeks` cold stratification insert ignore into journalissuearticles values(GP = 43.7, GV = 4.2); and 4 weeks` warm stratification + 8 weeks` cold stratification insert ignore into journalissuearticles values(GP = 45.8, GV = 4.3); were the most efficient in promoting germination performance, as well as dormancy removal. In the second study, the effects of storage at +4 ºC and -5 ºC for 12 months on the quality of pretreated seeds were investigated. Seeds from each provenance stored at +4 ºC were germinated during storage, while seeds stored at -5 ºC had high germination parameters after storage. In the third study, dormant seeds stored at -5 ºC and +4 ºC for 12 months were subjected to stratification insert ignore into journalissuearticles values(4 weeks of warm + 4 weeks of cold stratification); and then germinated at 30/20 ºC, 20 ºC and 25/4 ºC. Germination performance was not reduced by storage temperature in any provenances but was significantly affected by germination temperature, and the 25/4 ºC regime always gave the highest germination parameters insert ignore into journalissuearticles values(GP = 60.8, GV = 6.6);.
Keywords : Fraxinus angustifolia subsp oxycarpa, seed dormancy, pretreatment, seed storage