In Vitro Propagation of Some New Banana Types (Musa spp.)

Authors : Hamide GÜBBÜK*, Mustafa PEKMEZCİ

Pages : 355-361

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Publication Date : 2004-10-01

Article Type : Research Paper

Abstract :Three newly selected superior banana types were used to study the effects of different cytokinin and auxins on shoot multiplication and rooting. Benzylaminopurine [insert ignore into journalissuearticles values(BAP); insert ignore into journalissuearticles values(5, 10, 20 and 30 µM);] and thidiazuron [insert ignore into journalissuearticles values(TDZ); insert ignore into journalissuearticles values(0.4, 1, 2 and 3 µM);] were tested alone and with 1 µM indoleacetic acid insert ignore into journalissuearticles values(IAA); for the propagation stage. We compared basal Murashige and Skoog insert ignore into journalissuearticles values(MS); medium, active charcoal insert ignore into journalissuearticles values(5 g l-1);, indole-3-butyric acid insert ignore into journalissuearticles values(IBA); and naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); for rooting. Shoot proliferation and elongation were significantly greater with TDZ than with BAP all 3 types. Furthermore, each cytokinin with IAA increased shoot proliferation and elongation more than their use alone. BAP below 20 µM or TDZ below 1 µM did not increase shoot proliferation, and BAP over 20 µM and TDZ over 2 µM suppressed shoot elongation. Charcoal alone was better for rooting than auxin treatments or MS medium alone. In conclusion, supplementation of 2 µM TDZ, and 1 µM IAA or 20 µM BAP and 1 µM IAA on MS medium, followed 5 g l-1 charcoal at the rooting stage were the best combinations for the in vitro propagation of banana types.
Keywords : Banana, Musa spp, tissue culture, thidiazuron, active charcoal