Detection and Characterization of Plum Pox Potyvirus (PPV) by DAS-ELISA and RT-PCR / RFLP Analysis in Turkey

Authors : Gülşen SERTKAYA, Çiğdem ULUBAŞ, Kadriye ÇAĞLAYAN

Pages : 213-220

View : 26 | Download : 6

Publication Date : 2003-08-01

Article Type : Research Paper

Abstract :This study was conducted to determine the presence of plum pox virus insert ignore into journalissuearticles values(PPV); insert ignore into journalissuearticles values(family Potyviridae, genus Potyvirus); in different regions of Turkey and to characterize PPV isolates by serological and molecular techniques, including ELISA and PCR/ RFLP. Thus, leaf samples from different stone fruit species insert ignore into journalissuearticles values(almond, apricot, nectarine, peach, plum and sour and sweet cherry); exhibiting various types of symptoms related to PPV were collected from different parts of the canopy from randomly selected orchards in the main stone fruit growing areas in Turkey, except for Malatya province. Polyclonal antibodies were used to detect the presence of PPV in the plant samples by serological assays insert ignore into journalissuearticles values(DAS-ELISA);. The following monoclonal antibodies insert ignore into journalissuearticles values(MAbs);; Mab 5B insert ignore into journalissuearticles values(Universal);, Mab 4DG5 insert ignore into journalissuearticles values(PPV-D: Dideron-specific);, Mab AL insert ignore into journalissuearticles values(PPV-M: Marcus-specific);, Mab EA24 insert ignore into journalissuearticles values(PPV-El Amar-specific); and Mab AC insert ignore into journalissuearticles values(PPV-C: Cherry-specific);, were used to identify the serotyping of PPV isolates. Reverse transcription-polymerase chain reaction insert ignore into journalissuearticles values(RT-PCR); assays and restriction fragment length polymorphism analysis of RT-PCR products were performed to characterize Turkish PPV isolates. The results of RT-PCR analyses using general primers were in complete agreement with the DAS-ELISA and DASI-ELISA results, showing that 2 of 52 stone fruit samples collected from apricots in Ankara province were infected with the M strain of PPV. This study confirmed the results of the previous work and demonstrated the presence of the PPV-M strain in apricots in Turkey.
Keywords : Enzyme linked immunosorbent assay, plum pox potyvirus, restriction analysis, reverse transcription polymerase chain reaction, sharka