Dessiccation Using Saturated Salt Solutions and Improvement Germination Rate of Walnut (Juglans regia L.) Somatic Embryos

Authors : Hatice DUMANOĞLU

Pages : 491-498

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Publication Date : 2000-08-01

Article Type : Research Paper

Abstract :Desiccation of somatic embryos under different air humidities conditioned by saturated salt solutions for different dura-tions; relationships between desiccation and germination or root formation in the dark; and effects of desiccation and gibberellic acid on germinating of `Su-2` walnut somatic embryonic embryo line were investigated. Survival of embryos was low insert ignore into journalissuearticles values(about 10%); or zero when loss of fresh weight was about 90% and dry matter was about 70%. The highest germinating percentages insert ignore into journalissuearticles values(13.3-23.3%); were obtained with about 40.4-77.3% of loss of fresh weight and 9.1-34.6% of dry matter of desiccated embryos in covered plates over saturated MgCl 2 .6H 2 O for 4 days, Mginsert ignore into journalissuearticles values(NO 3 ); 2 .6H 2 O for 5 days or NaCl for 5-6 days, and with uncovered plates over ZnSO 4 .7H 2 O for 6-7 days or NH 3 SO 4 for 6-8 days. The highest rooting percentage insert ignore into journalissuearticles values(80.9%); in the dark were obtained with 56.2% of loss of fresh weight and 16.8% of dry matter of desiccated embryos in covered plates over saturated MgCl 2 .6H 2 O for 4 days. To improve germination, both desiccation and GA 3 treatments were more effective than only desiccation or GA 3 treatments on germination. Germinating was best insert ignore into journalissuearticles values(46.0%); with desiccation followed by incubation in the dark for 15 days prior to being transferred to a medium containing 9 mg/l GA 3 in the light.
Keywords : Turk J Agric For, 24, 2000, , 491 498 Turk J Agric For, vol 24, iss 4