Effects of Different Media, Type and Concentrations of Auxin on Callus Induction and Plant Regeneration from Young Inflorescences of Yellow Bluestem (Bothriochloa ischaemum(L.) Keng)

Authors : Ersin CAN

Pages : 221-230

View : 27 | Download : 5

Publication Date : 2000-04-01

Article Type : Research Paper

Abstract :This study was conducted to determine the effects of different media, auxin types and concentrations on callus induction and plant regeneration from young inflorescences of 15 ecotypes of yellow bluestem insert ignore into journalissuearticles values( Bothriochloa ischaemum);. The young inflorescences were cultured on two different basal media insert ignore into journalissuearticles values(LS and SH); containing different concentrations insert ignore into journalissuearticles values(2, 4, 6, and 8 mg/l); of two different auxins insert ignore into journalissuearticles values(2, 4-D and Dicamba);. The results showed that the ecotypes were significantly different from each other in callus induction rate, callus weight per dish and regenerate number per inflorescence segment. Depending on the ecotypes, mean callus induction rate varied from 9.7% 51.6%, mean callus weight per dish from 95.6 to 414.6 mg and number of regenerates per inflorescence segment from 0 to 2.2. LS medium resulted in better callus induction, and callus weight and regeneration than SH medium. While the media with 2,4-D produced higher callus induction and callus weight than those with dicamba, the media supplemented with dicamba produced more regenerates per inflorescence segment than those with 2,4-D. The highest value for both callus induction and callus weight was obtained at 4 mg/l 2,4-D concentration. However, the highest plant regeneration was recorded at 8 mg/l dicamba concentration.
Keywords : Turk J Agric For, 24, 2000, , 221 230 Turk J Agric For, vol 24, iss 2