Obtaining Plants From Seedless x Seedless Grape Crosses Using Embryo Culture

Authors : Semih TANGOLAR, Sinan ETİ, Serpil GÖK, Fuat ERGENOĞLU

Pages : 935-942

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Publication Date : 1999-09-01

Article Type : Research Paper

Abstract :This experiment was planned to research the possibilities of the use of embryo culture on seedless grape breeding. Samples of Sultani çekirdeksiz insert ignore into journalissuearticles values(Thompson Seedless);, Ergin çekirdeksizi, 3A-261, Perlette, Pembe çekirdeksiz, 2B-56, Flame Seedless and King`s Ruby seedless grape varieties were used. In the first year of experiment, pollen viability and germination, amount of pollen production and their morphological homogenity levels were determined. In the second year, plants were tried to obtain from seedless x seedless crossess by embryo culture. The results obtained in the experiment showed that all of the varieties had enough pollen viability and germination capacity. In addition, the amount of pollen production and their morphological homojenity levels were sufficient. Plants were obtained succesfully from hybrid combinations by embryo culture. Germination period of embryos were ranged between 2.5 days insert ignore into journalissuearticles values(Ergin çekirdeksizi x Perlette); and 9 days insert ignore into journalissuearticles values(Flame seedless x Perlette);. The rate of germination was found over 77.1% insert ignore into journalissuearticles values(in Flame Seedless x Perlette);. Percent of whole plant obtained from germinated embryos was zero in Ergin çekirdeksizi x Perlette while 78.9% in Perlette x Pembe çekirdeksiz combinations.
Keywords : Turk J Agric For, 23, 1999, , 935 942 Turk J Agric For, vol 23, iss EK4