Determination of fatty acids and volatile compounds in fruits of rosehip(Rosa L.) species by HS-SPME/GC-MS and Im-SPME/GC-MS techniques

Authors : ZEHRA TUĞBA MURATHAN, MOZGAN ZARIFIKHOSROSHAHI, NESİBE EBRU KAFKAS

Pages : 269-279

View : 16 | Download : 6

Publication Date : 2016-04-01

Article Type : Research Paper

Abstract :In this study, we aimed to compare fatty acid and volatile compound compositions of four rosehip species, namely Rosa pimpinellifolia, R. Villosa, R. Canina, and R. Dumalis, by gas chromatography with flame ionization detector insert ignore into journalissuearticles values(GC/FID); and headspace and immersion solid-phase microextraction gas chromatography-mass spectrometry insert ignore into journalissuearticles values(HS-SPME/GC-MS and Im-SPME/GC-MS); techniques. The total lipid contents in fruits of the rosehip species varied from 5.83% insert ignore into journalissuearticles values(R. Villosa); to 7.84% insert ignore into journalissuearticles values(R. Dumalis);. A total of 21 fatty acids were detected and quantified. In all species, except R. Canina, polyunsaturated fatty acids insert ignore into journalissuearticles values(PUFAs); predominated over saturated fatty acids insert ignore into journalissuearticles values(SFAs); and monounsaturated fatty acids insert ignore into journalissuearticles values(MUFAs);. Palmitic acid is the major SFA in R. Villosa insert ignore into journalissuearticles values(5.50%);, R. Canina insert ignore into journalissuearticles values(8.27%);, and R. Dumalis insert ignore into journalissuearticles values(7.46%);. Oleic acid is the most abundant MUFA, and linoleic and α-linolenic acids are the most abundant PUFAs. Sixty-two volatile compounds were detected by the HS-SPME/GC-MS technique, and 54 volatile compounds were determined by the Im-SPME/GC-MS technique. Fifty-three volatile components of rosehips have been detected for the first time in this study. While 19 acids, 9 aldehydes, 6 ketones, 18 alcohols, 5 esters, 2 terpenes, and 2 phenols were identified by HS-SPME/GC-MS, 20 acids, 5 aldehydes, 8 ketones, 13 alcohols, 5 esters, 1 terpene, and 2 phenols were identified by Im-SPME/GC-MS. The HS-SPME/GC-MS technique allowed identification of a larger number of volatile compounds and thus is more efficient than the Im-SPME/GC-MS technique.
Keywords : Fatty acid, HS SPME GC MS, Im SPME GC MS, rosehip, volatiles