Genetic diversity within Turkish watermelon [Citrullus lanatus (Thunb.)Matsumura & Nakai] accessions revealed by SSR and SRAP markers

Authors : İlknur SOLMAZ, Yildiz KAÇAR, Nebahat SARI, Özhan ŞİMŞEK

Pages : 407-419

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Publication Date : 2016-06-01

Article Type : Research Paper

Abstract :The genetic diversity of Turkish watermelon genotypes selected from the watermelon genetic resource collection was evaluated by simple sequence repeat insert ignore into journalissuearticles values(SSR); and sequence-related amplified polymorphism insert ignore into journalissuearticles values(SRAP); markers. Most of the accessions were collected from various geographical regions of Turkey. Different Citrullus species, wild relatives, foreign landraces, open pollinated insert ignore into journalissuearticles values(OP);, and commercial hybrid cultivars were also assessed for genetic relatedness. Fourteen SSR primers and 31 SRAP primer combinations were used in the experiment. Both SSR markers insert ignore into journalissuearticles values(100%); and SRAP markers insert ignore into journalissuearticles values(97.3%); generated high polymorphisms. Based on the SSR and SRAP data, the genetic similarity coefficients were calculated, and dendrograms were constructed using the unweighted pair-group method with arithmetic average insert ignore into journalissuearticles values(UPGMA);. Cluster and principle coordinate analyses indicated that Citrullus lanatus var. Lanatus subspecies genotypes collected from the different regions of Turkey were closely genetically related. Overall, our results displayed low genetic variability within the Turkish watermelon germplasm in contrast with their high morphological diversity.
Keywords : Citrullus lanatus, genetic resources, microsatellite, molecular markers, SRAP