Improvement of rebaudioside A diterpene glycoside content in Stevia rebaudiana Bertoni using clone selection

Authors : Begüm KAPLAN, Kenan TURGUT

Pages : 232-240

View : 12 | Download : 6

Publication Date : 2019-04-01

Article Type : Research Paper

Abstract :This study, conducted in Antalya, Turkey, between 2014 and 2016, aimed to determine genotypes with a high amount of rebaudioside A insert ignore into journalissuearticles values(reb A); and high reb A/stevioside insert ignore into journalissuearticles values(stv); ratio in Stevia rebaudiana Bertoni, and reveal superior candidate varieties via the clone selection method. The study started with 200 genotypes in the first year, followed by the stem cuttings of 40 genotypes selected in the second year, in accordance with the obtained data, which were grown in rows insert ignore into journalissuearticles values(A clones);, and the 10 genotypes insert ignore into journalissuearticles values(B clones); selected from the clones in the third year, which were grown in 3 replications according to a completely randomized block design. In accordance with the criteria for the evaluation of the results, 4 plants with superior characteristics were selected insert ignore into journalissuearticles values(C clones);. Genotype 82 was regarded as a candidate with superior characteristics in terms of the amount of reb A content insert ignore into journalissuearticles values(7.95%, 7.77%, and 6.37%, respectively); and reb A/stv ratio insert ignore into journalissuearticles values(1.48, 1.20, and 1.48); over the 3 years. Genotype 109 was noteworthy for stv alone. Genotype 133 was considered as not only high in reb A insert ignore into journalissuearticles values(8.47%, 6.81%, and 6.40%, respectively);, but also as an industrial material suitable for processing and production, as the agronomic properties were above average during the study. Genotype 196 was determined to be high in terms of the amount of reb A + stv insert ignore into journalissuearticles values(18.31%, 12.54%, and 13.58, respectively); and reb A insert ignore into journalissuearticles values(7.57%, 6.46%, and 6.2%, respectively);. Therefore, these were selected as clonal variety candidates.
Keywords : Breeding, clone selection, rebaudioside A, Stevia rebaudiana, stevioside