Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

Authors : Handan BALTA, Özge KARAKAŞ METİN, Funda ŞENTÜRK AKFIRAT, Fahriye ERTUĞRUL, Semra HASANÇEBİ

Pages : 371-379

Doi:10.3906/biy-1311-8

View : 23 | Download : 6

Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat insert ignore into journalissuearticles values(Triticum aestivum L.); in this study. DNA isolated from the selected yellow rust tolerant and susceptible F2 individuals derived from a cross between Izgi2001 insert ignore into journalissuearticles values(resistant); and ES14 insert ignore into journalissuearticles values(susceptible); at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG insert ignore into journalissuearticles values(133 bp); was identified and presented in the resistant parent and resistant F2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC133bp marker by the PCR walking method to design SCAR markers for wheat breeding programs.
Keywords : Key words AFLP, bread wheat, disease resistance, SCAR, yellow rust