Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Authors : Liliana JARDA, Anca BUTIUC-KEUL, Maria HÖHN, Andrzej PEDRYC, Victoria CRISTEA

Pages : 21-30

Doi:10.3906/biy-1303-20

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Publication Date : 2014-12-01

Article Type : Research Paper

Abstract :Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.
Keywords : Key words ISSR, SSR markers, Dianthus giganteus subsp banaticus, ex situ conservation, in vitro culture, somaclonal variability