Characterization, antifungal activity, and cell immobilization of a chitinase from Serratia marcescens MO-1

Authors : Sezer OKAY, Murat ÖZDAL, Esabi Başaran KURBANOĞLU

Pages : 639-644

Doi:10.3906/biy-1208-45

View : 15 | Download : 4

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Chitinases have the potential to control many pathogen species, such as fungi containing chitin on their cell walls. In the present study, chitinase from novel isolate Serratia marcescens MO-1, isolated from Poecilimon tauricola insert ignore into journalissuearticles values(Orthoptera: Tettigoniidae); in Turkey, was investigated. The optimum pH and temperature for the chitinase activity were found to be pH 7.0 insert ignore into journalissuearticles values(36.6 U/mL); and 50 °C insert ignore into journalissuearticles values(37.1 U/mL);, respectively. Moreover, the activity was still high in acidic insert ignore into journalissuearticles values(23.0 U/mL at pH 3.0); and basic insert ignore into journalissuearticles values(17.3 U/mL at pH 11.0); conditions as well as at lower insert ignore into journalissuearticles values(29.5 U/mL at 20 °C); and higher insert ignore into journalissuearticles values(28.7 U/mL at 80 °C); temperatures. The enzyme was highly thermotolerant, keeping 63.7% insert ignore into journalissuearticles values(23.5 U/mL); of its activity after incubation at 90 °C for 15 min. Antifungal activity of the chitinase against Alternaria citri, Fusarium oxysporum, Trichoderma harzianum, Aspergillus niger, and Rhizopus oryzae was determined. In addition, chitinase production by immobilized S. marcescens MO-1 cells was monitored over 10 days; the enzyme activity was found to be 36–41 U/mL during this period. Our results showed that the chitinase from S. marcescens MO-1 seems to be valuable in terms of its biotechnological applications; it can be produced using immobilized cells and can be used against fungal pathogens as an alternative to chemical pesticides.
Keywords : Key words Serratia marcescens, chitinase, cell immobilization, antifungal activity