Establishment and optimization of cell growth in suspension culture of Papaver bracteatum: a biotechnology approach for thebaine production

Authors : Reza FARJAMINEZHAD, Nasser ZARE, Rasool ASGHARI-ZAKARIA, Manoochehr FARJAMINEZHAD

Pages : 689-697

Doi:10.3906/biy-1304-54

View : 15 | Download : 6

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Iranian poppy insert ignore into journalissuearticles values(Papaver bracteatum); is an important medicinal plant that is the main source of the opium alkaloids codeine, morphine, and thebaine. To establish an efficient protocol for cell suspension culture and growth, the effects of different plant growth regulators insert ignore into journalissuearticles values(2,4-D, NAA, BAP, and kinetin); on callus induction and cell suspension culture of Iranian poppy were evaluated. The maximum percentage of callus induction insert ignore into journalissuearticles values(86.67%); and fresh weight of callus were obtained in MS medium supplemented with 1 or 2 mg/L 2,4-D, 0.1 or 0.2 mg/L kinetin and 15 mg/L ascorbic acid. The addition of 15 mg/L ascorbic acid to the medium was effective in reducing browning of explants and calli. In cell suspension cultures, MS medium supplemented with 1 mg/L NAA, 1 mg/L BAP, and 15 mg/L ascorbic acid gave maximum cell growth and proliferation compared to other treatments evaluated. Among the auxins, NAA was more effective than 2,4-D for growth of P. bracteatum cell suspension cultures. However, there was no statistically significant difference between kinetin and BAP. The thebaine yield of P. bracteatum cell suspension at 12 days after culture was 4.67 µg/g dry weight, which was significantly lower than that at 16 days insert ignore into journalissuearticles values(10.76 µg/g dry weight);.
Keywords : Key words Cell suspension culture, Papaver bracteatum, secondary metabolites, thebaine