Characterization of a new isolate of Malacosoma neustria nucleopolyhedrovirus (ManeNPV) from Turkey

Authors : İsmail DEMİR, Remziye NALÇACIOĞLU, Lida MOHAMMAD GHOLİZAD, Zihni DEMİRBAĞ

Pages : 385-391

Doi:10.3906/biy-1209-24

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Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :The lackey moth, Malacosoma neustria insert ignore into journalissuearticles values(Linnaeus, 1758);, a worldwide pest, causes extensive economic losses on particularly hazelnut, Prunus, Quercus, Populus, and Salix trees. In this study, a local nucleopolyhedrovirus insert ignore into journalissuearticles values(NPV); was isolated from the larvae of M. neustria in the northeast of Turkey. It was named ManeNPV-T2. Electron microscopic observations showed that the polyhedra of the new isolate contain several virions with multiple nucleocapsids packaged within a single viral envelope. Restriction endonuclease analysis of ManeNPV-T2 DNA indicated that it has a different restriction profile compared to the previous Turkish and Latvian isolates. The phylogenetic analysis of the amplified polh sequence of ManeNPV-T2 showed its relation to the other NPVs from Malacosoma species. Mortality values for third instar M. neustria larvae ranged from 48% to 100% according to the concentration of polyhedral occlusion bodies. Based on screening tests, the LD50 value of the new isolate was calculated as 1.34 × 104 occlusion bodies per larvae. Consequently, ManeNPV appears to be a promising microbial control agent for biocontrol of M. neustria.
Keywords : Key words Malacosoma neustria, nucleopolyhedrovirus, characterization, ManeNPV, insect virus