Pomegranate (Punica granatum L.) reduces endoplasmic reticulum stress induced by renal ischemia/reperfusion injury in rat

Authors : Khadijeh HASHEMI, Kazem PARIVAR, Samira FAZLIFAR, Alireza HAGHPARAST, Mehrdad MOHRI

Pages : 464-471

Doi:10.3906/biy-1211-17

View : 12 | Download : 4

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Ischemia/reperfusion insert ignore into journalissuearticles values(I/R); is one of the main causes of acute renal failure, leading to generation of reactive oxygen species insert ignore into journalissuearticles values(ROS); and ensuing oxidative stress, which results in unfolded/misfolded protein accumulation and its dependent response pathways, generally known as unfolded protein response insert ignore into journalissuearticles values(UPR);, in the endoplasmic reticulum insert ignore into journalissuearticles values(ER);. We studied the effects of renal I/R on the expression of ER-stress genes, as well as concomitant effects of oral pretreatment with pomegranate insert ignore into journalissuearticles values(Punica granatum L.); pith and carpellary membrane insert ignore into journalissuearticles values(PPCM);. An in vivo model of rat kidney I/R followed by conventional and real-time RT-PCR was used to evaluate the modulation of GRP78 and XBP1 as central UPR and ER-stress markers. Ischemia followed by reperfusion insert ignore into journalissuearticles values(60 and 150 min, respectively); resulted in decreased antioxidant properties of plasma insert ignore into journalissuearticles values(lowered ferric reducing ability of plasma [FRAP] value); and GRP78 transcript levels. Oral administration of PPCM aqueous extract for 10 days with or without ischemia insert ignore into journalissuearticles values(PPCM and PPCM/Isc groups, respectively); was able to further decrease the GRP78 expression, while it increased the FRAP value. PPCM pretreatment also reduced the XBP1 splicing in the PPCM/Isc group compared to the Isc group. These results suggest that UPR is activated during oxidative insults induced by I/R, while PPCM can reduce I/R-induced ER stress in rat kidneys via antioxidant defense mechanisms.
Keywords : Key words Antioxidant, ER stress, GRP78, pomegranate, unfolded protein response, XBP1