Concentration of sodium dodecyl sulfate used in occlusion body extraction affects Spodoptera littoralis nucleopolyhedrovirus biological activity

Authors : Hilal SUSURLUK, Umut TOPRAK, Mehmet Oktay GÜRKAN

Pages : 171-175

Doi:10.3906/biy-1204-50

View : 13 | Download : 5

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :Efficacy of a baculovirus as a biological control agent is evaluated according to biological parameters, median lethal dose insert ignore into journalissuearticles values(LD50); of the virus, and median lethal time insert ignore into journalissuearticles values(LT50); of infected larvae. Sodium dodecyl sulfate insert ignore into journalissuearticles values(SDS); is commonly used in concentrations of 0.1%-1% to extract infective units of the baculoviruses, occlusion bodies insert ignore into journalissuearticles values(OBs);, from infected cadavers. Although several studies have shown disruption of OB structure by SDS, the effects of SDS concentrations on baculovirus biological parameters are not well known. Indeed, it is essential to detect the optimum SDS concentration for OB extraction to compare the activity of baculoviruses accurately. In our study, the effects of SDS insert ignore into journalissuearticles values(0.1%-2%); on baculoviral biological activity were examined using Spodoptera littoralis nucleopolyhedrovirus in third instar Spodoptera littoralis insert ignore into journalissuearticles values(Lepidoptera: Noctuidae); larvae. Use of 1% SDS in OB extraction caused the lowest LD50, while higher concentrations than 1% significantly increased the LD50. The differences between LT50 values at 0.1%-1% SDS were found to be insignificant; however, higher concentrations insert ignore into journalissuearticles values(>1.0%); caused significant loss of biological activity. Therefore, 0.1%-1.0% SDS could be used for OB extraction; however, evaluation of LD50 and LT50 values together suggests that 1% SDS provides the best biological activity. The mode of action of SDS on baculovirus biological activity is discussed further.
Keywords : Key words Sodium dodecyl sulfate, baculovirus, occlusion body, lethal dose, lethal time