Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg × Sultani progeny

Authors : Murat AKKURT, Atilla ÇAKIR, Mina SHİDFAR, Filiz MUTAF, Gökhan SÖYLEMEZOĞLU

Pages : 101-105

Doi:10.3906/biy-1206-31

View : 15 | Download : 7

Publication Date : 2013-12-01

Article Type : Research Paper

Abstract :The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg insert ignore into journalissuearticles values(seeded); × Sultani insert ignore into journalissuearticles values(seedless); progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction insert ignore into journalissuearticles values(PCR); using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.
Keywords : Key words Grapevine, marker assisted selection, seedlessness