Genetic variability in Grapevine virus A from Vitis vinifera L. × Vitis labrusca L. in Sichuan, China

Authors : Jianhui WANG, Dehui XI, Jianjun LIU, Keling CHEN, Hongwen LI, Xiao LIU, Shu YUAN, Sezai ERCİŞLİ, Honghui LIN

Pages : 542-551

View : 11 | Download : 4

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Grapevine virus A insert ignore into journalissuearticles values(GVA); has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants insert ignore into journalissuearticles values(Vitis vinifera L. × Vitis labrusca L.);. The results of restriction fragment length polymorphism insert ignore into journalissuearticles values(RFLP); studies on the polymerase chain reaction insert ignore into journalissuearticles values(PCR); products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that yielded different PCR-RFLP profiles were preferentially chosen for sequencing and were designated as variants. Furthermore, a phylogenetic study based on the analysis of the GVA coat protein genes and RNA silencing suppressor genes showed that the 40 variants obtained from the 15 GVA isolates represented 4 clades, designated as molecular groups I, II, III, and IV, respectively. The variant-specific PCR detection results indicated that the parts of the tested grape plants were specifically infected by mild variants.
Keywords : Key words Vitis vinifera L × Vitis labrusca L, Grapevine virus A, PCR RFLP, cloning and sequencing, RT PCR