Genotoxicity of bovine bone-derived microhydroxyapatite (Boneplus-xs)

Authors : Mehmet TOPAKTAŞ, Erman Salih İSTİFLİ, Mehmet BÜYÜKLEYLA, Hasan Basri İLA

Pages : 568-573

View : 13 | Download : 4

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :The aim of this study was to evaluate the genotoxic effect of a bovine bone-derived hydroxyapatite insert ignore into journalissuearticles values(HA);, Boneplus-xs insert ignore into journalissuearticles values(B-P-XS);. The genotoxicity of B-P-XS was investigated by examining the potential of the xenograft insert ignore into journalissuearticles values(B-P-XS); to induce gene mutation insert ignore into journalissuearticles values(evaluated using the Ames test);, in vitro chromosome aberration insert ignore into journalissuearticles values(CA);, and sister chromatid exchange insert ignore into journalissuearticles values(SCE); with tests in cultured human peripheral lymphocytes. Counts of reverse mutations in Salmonella typhimurium TA98 and TA100 strains were not found to be significantly increased after treatment with B-P-XS insert ignore into journalissuearticles values(P > 0.05);. The presence of B-P-XS in peripheral blood culture for 24 and 48 h did not lead to a significant increase in the frequency of CAs insert ignore into journalissuearticles values(P > 0.05); and SCEs insert ignore into journalissuearticles values(P > 0.05); when compared to the solvent control insert ignore into journalissuearticles values(dimethyl sulfoxide);. When compared with the control group insert ignore into journalissuearticles values(untreated);, B-P-XS significantly decreased the mitotic index; however, the decrement was not statistically significant when compared with the solvent control in any of the concentrations and treatment times insert ignore into journalissuearticles values(P > 0.05);. In addition, no significant changes in the proliferation index were observed after treatment with B-P-XS when compared to both the control and the solvent control. As a result of this study, we suggest that HA microparticles greater than 200 µm do not constitute a genotoxic risk for patients undergoing dental surgery.
Keywords : Key words Hydroxyapatite, chromosome aberration, sister chromatid exchange, Ames test