Genotoxic and mutagenic effects of aqueous extract from aerial parts of Achillea teretifolia

Authors : Dilek AKYIL, Sedat OKTAY, Recep LİMAN, Yasin EREN, Muhsin KONUK

Pages : 441-448

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Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Genotoxic and mutagenic effects of aqueous extract from aerial parts of Achillea teretifolia insert ignore into journalissuearticles values(At-ext); were investigated using both Allium cepa root meristematic cells and a short-term mutation assay in Salmonella typhimurium with TA98 and TA100 strains in the presence or absence of S9 mix, respectively. In the Allium tests, EC50 value was determined as 50 g/L, and 0.5 × EC50, EC50, and 2 × EC50 concentrations of At-ext were applied to onion tuber roots. Methyl methane sulfonate insert ignore into journalissuearticles values(MMS, 10 ppm); and distilled water were used as positive and negative controls, respectively. The mitotic index insert ignore into journalissuearticles values(MI); increased in all concentrations in comparison to control at each exposure time except 25 and 50 g/L at 12 h. While stickiness, chromosome laggards, bridges, and disturbed anaphase-telophase were observed in anaphase-telophase cells, pro-metaphase, c-metaphase, polyploidy, and binuclear cells were observed in other cells. It was determined that At-ext have no mutagenic effect on S. typhimurium TA98 and TA100 strains in the presence or absence of S9 mix. The results were statistically analyzed in SPSS using Duncan`s multiple range test. These results indicate that At-ext has genotoxic activity in A. cepa root meristematic cells.
Keywords : Key words Allium, Ames, chromosome aberration, mutagenicity