A study on the characterization and pathogenicity of bacteria from Lymantria dispar L. (Lepidoptera: Lymantriidae)

Authors : İsmail DEMİR, Emine ERYÜZLÜ, Zihni DEMİRBAĞ

Pages : 459-468

View : 15 | Download : 5

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Gypsy moth, Lymantria dispar L. insert ignore into journalissuearticles values(Lepidoptera: Lymantriidae);, is one of the most serious pests affecting fruit, forest, industrial, and foliage plants worldwide. The aim of this study was to isolate and characterize pathogenic bacteria from this pest and determine their pathogenicity. L. dispar larvae were collected from various populations in the forest areas of the Black Sea region of Turkey in May and June of 2008 and 2009, and 9 culturable bacterial isolates were obtained from these larvae. Morphological and physiological characteristics of bacterial isolates were determined by conventional and routine techniques, and biochemical properties and metabolic enzyme profiles were revealed using API20E and Phoenix 1000A panel test systems. Additionally, a 16S rRNA gene sequence analysis was performed to identify the isolates at the molecular level and to query against the NCBI genetic database. Consequently, 7 of the bacterial isolates were determined and characterized at the species level while the rest were characterized at the genus level. From the results of all tests, the isolates were identified as Pantotea agglomerans insert ignore into journalissuearticles values(Lyd1);, Klebsiella sp. insert ignore into journalissuearticles values(Lyd2);, Acinetobacter calcoaceticus insert ignore into journalissuearticles values(Lyd3);, Serratia marcescens insert ignore into journalissuearticles values(Lyd4);, Erwinia sp. insert ignore into journalissuearticles values(Lyd5);, Bacillus thuringiensis insert ignore into journalissuearticles values(Lyd6);, B. thuringiensis insert ignore into journalissuearticles values(Lyd7);, B. thuringiensis insert ignore into journalissuearticles values(Lyd8);, and B. thuringiensis insert ignore into journalissuearticles values(Lyd9);. Of all bacterial isolates tested for insecticidal activity, 2 of the B. thuringiensis isolates insert ignore into journalissuearticles values(Lyd6 and Lyd9); were found to cause the highest insecticidal activity insert ignore into journalissuearticles values(90%); within 10 days of application. Furthermore, 100% insecticidal activity was observed with a crystal-spore mixture of the same isolates against Lymantria dispar larvae within 10 days.
Keywords : Key words Lymantria dispar, culturable bacterial flora, insecticidal activity, Bacillus thuringiensis