Determination of genetic relationships among Velezia L. Caryophyllaceae); species using RAPD markers

Authors : İlham Eröz POYRAZ, Emel SÖZEN, Ebru ATAŞLAR, İsmail POYRAZ

Pages : 293-302

View : 16 | Download : 7

Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :Random amplified polymorphic DNA insert ignore into journalissuearticles values(RAPD); markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplified bands were obtained using 14 RAPD primers. The polymorphism in RAPD markers was high insert ignore into journalissuearticles values(98.61%); and was sufficient to distinguish each species. The degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean insert ignore into journalissuearticles values(UPGMA);. Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that the RAPD technique could be successfully used for the determination of genetic relationships among Velezia species and for species identification. Furthermore, it can be efficiently employed in future studies to provide preliminary data for conservation of endangered Velezia species.
Keywords : Key words Genetic relationships, RAPD PCR, Velezia L, Turkey