Thermostable a-amylase from moderately halophilic Halomonas sp. AAD21

Authors : Kamil Serkan UZYOL, Berna SARIYAR AKBULUT, Aziz Akın DENİZCİ, Dilek KAZAN

Pages : 327-338

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Publication Date : 2012-12-01

Article Type : Research Paper

Abstract :The moderately halophilic Halomonas sp. strain AAD21, which produces extracellular thermostable a-amylase, was isolated from the Çamaltı Saltern area located in İzmir, Turkey. NaCl, carbon, and nitrogen sources in the growth medium were optimized to enhance a-amylase yield. The highest enzyme yield was measured in the presence of 20% NaCl with peptone as the nitrogen and starch as the carbon sources in the fermentation broth. This microorganism was also found to utilize waste potato peel as a carbon source for a-amylase production. Concentrations of carbon and nitrogen sources were optimized using a statistical approach, and a-amylase activity increased from 4.07 U mL-1 min-1 to 26.25 U mL-1 min-1. Maximum a-amylase production was achieved at the end of 48 h of growth in the presence of 20% NaCl, 4.12% starch, 1.0% peptone, 0.2% KCl, 2% MgSO4.7H2O, and 0.03% trisodium citrate pentahydrate. The optimum pH and temperature of the a-amylase were found to be 7.0 and 50 °C, respectively. The a-amylase synthesized by Halomonas sp. AAD21 was also thermostable. Crude a-amylase did not lose its original activity after 2 h of incubation at 50 °C and 60 °C, and it retained 70% of its original activity after 120 min of incubation at 90 °C.
Keywords : Key words Moderate halophile, Halomonas sp AAD21, a amylase, thermostable, optimization