A comparative evaluation of antioxidant and anticancer activity of essential oil from Origanum onites (Lamiaceae) and its two major phenolic components

Authors : Aysun ÖZKAN, Ayşe ERDOĞAN

Pages : 735-742

View : 17 | Download : 5

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :The objectives of the present study were to determine the effects of essential oil from Turkish oregano insert ignore into journalissuearticles values(Origanum onites L.); and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective insert ignore into journalissuearticles values(antioxidant); effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 insert ignore into journalissuearticles values(Hep G2); cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus insert ignore into journalissuearticles values(hydrodistillation); and its components were analyzed by gas chromatography/mass spectrometry insert ignore into journalissuearticles values(GC/MS);. The essential oil of O. onites decreased cancer cell viability at higher concentrations. The essential oil was found to be less cytototoxic insert ignore into journalissuearticles values(IC50: 149.12 µg/mL); than carvacrol and thymol insert ignore into journalissuearticles values(IC50: 53.09 and 60.01 µg/mL, respectively); for Hep G2 cells. On the other hand, with the O. onites essential oil, carvacrol and thymol protected the cells against H2O2-induced cytotoxicity when the cells were preincubated with the oil and its components at a lower concentration insert ignore into journalissuearticles values(
Keywords : Key words O onites, essential oil, antioxidant, anticancer