In vitro propagation of Diyarbakır watermelons and comparison of direct-seeded and transplanted watermelon

Authors : Veysi OKUMUŞ, Vedat PİRİNÇ, Ahmet ONAY, Davut BAŞARAN

Pages : 601-610

View : 13 | Download : 6

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :A rapid protocol using shoot tip explants for micropropagation of Diyarbakır watermelon [Citrullus lanatus insert ignore into journalissuearticles values(Thunb.); Matsum. & Nakai] types Sürme, Beyazkış and Karakış was achieved. Shoot tips from 5-day-old in vitro germinated seedlings were cultured on shoot regeneration medium for 3 weeks. The effects of the different concentrations of benzyl adenine insert ignore into journalissuearticles values(BA); and carbohydrate types on shoot proliferation were examined. The results confirmed that a range of 0.5-1.0 mg/L of BA was almost equally effective in promoting the shoot length of cultures in the 3 genotypes. Sucrose, in the presence of BA, was superior to other carbohydrates for the 3 genotypes studied in terms of the number of proliferated shoots and the average shoot length obtained. The highest percentage of rooting was achieved when medium supplemented with 1.0 mg/L of indole-3-butyric acid insert ignore into journalissuearticles values(IBA); was used for the 3 genotypes. The highest frequency of acclimatized plantlets for the 3 genotypes was 85%, 85%, and 90%, respectively, in sterile compost, when the shoots of Beyazkış, Karakış, and Sürme were rooted in the IBA-supplemented rooting treatments. The Sürme genotype produced a significantly higher yield and mean fruit weight than the Beyazkış and Karakış genotypes in both direct-seeded and transplanted watermelons. The main soluble sugar of the experimental types was separated, identified, and quantified using high performance liquid chromatography insert ignore into journalissuearticles values(HPLC);. Fructose was found to be the most abundant sugar and was highly detected in Beyazkış for the seeded watermelon and in Karakış for the transplanted watermelon. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbakır watermelon for use in breeding lines.
Keywords : Key words Watermelon, regeneration, tissue culture, yield, fruit