Molecular cloning, characterization, and expression analysis of a gene encoding a Ran binding protein (RanBP) in Cucumis melo L.

Authors : Mehmet Cengiz BALOĞLU, Florence Negre ZAKHAROV, Hüseyin Avni ÖKTEM, Ayşe Meral YÜCEL

Pages : 387-397

View : 16 | Download : 6

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :Ran binding proteins insert ignore into journalissuearticles values(RanBPs); are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated insert ignore into journalissuearticles values(Cucumis melo L.); using the RACE insert ignore into journalissuearticles values(rapid amplification of cDNA ends); method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids insert ignore into journalissuearticles values(22.2 kDa protein);, was characterized insert ignore into journalissuearticles values(GenBank accession no: EU853459);. The predicted amino acid sequence of CmRanBP was found to be 70% identical to VvRanBP, PtRanBP, and RcRanBP from Vitis vinifera, Populus trichocarpa, and Ricinus communis, respectively. Within the RanBD insert ignore into journalissuearticles values(Ran binding domain);, 5 highly conserved motifs and 1 Ran binding motif were found in all members of the RanBP gene family from various plant species. Expression profiles of the CmRanBP gene in different tissues under high temperature stress were also investigated by semiquantitative RT-PCR. The CmRanBP gene was expressed in a similar manner in the roots, leaves, and stems at 25 °C as a control environment. However, when the temperature was raised to 38 °C and 40 °C, expression levels of the CmRanBP gene were significantly insert ignore into journalissuearticles values(P < 0.05); increased in the root, leaf, and stem tissues. We show here for the first time that the CmRanBP gene expression was correlated with heat stress responses.
Keywords : Key words Melon Cucumis melo L, , Ran binding protein RanBP, , gene cloning, gene expression, heat stress