Characterisation and antimicrobial activity of Sophora alopecuroides L. var. alopecuroides alkaloid extracts

Authors : Nurgün KÜÇÜKBOYACI, Semiha ÖZKAN, Nezaket ADIGÜZEL, Fatma TOSUN

Pages : 379-385

View : 14 | Download : 5

Publication Date : 2011-12-01

Article Type : Research Paper

Abstract :The aerial parts and seeds of Sophora alopecuroides L. var. alopecuroides growing in Turkey were investigated for their alkaloid compositions and antimicrobial activities. The alkaloid extracts were analysed by capillary gas chromatography-mass spectrometry insert ignore into journalissuearticles values(GC-MS);. The main components were identified as sophoridine insert ignore into journalissuearticles values(33.07%);, matrine insert ignore into journalissuearticles values(24.03%);, and sophocarpine insert ignore into journalissuearticles values(8.01%); in the aerial parts, and matrine insert ignore into journalissuearticles values(36.67%); and sophoridine insert ignore into journalissuearticles values(34.53%); in the seeds of the plant. The alkaloid extracts were also evaluated for their in vitro antibacterial and antifungal activities using microdilution techniques. The alkaloid extract of the aerial parts of S. alopecuroides var. alopecuroides presented significant activity against Staphylococcus aureus and Bacillus subtilis while the alkaloid extract of the seeds displayed good activity against Pseudomonas aeruginosa and Bacillus subtilis with minimum inhibitory concentrations insert ignore into journalissuearticles values(MICs); of 62.5 µg/mL. In the antifungal assay, the alkaloid extract of the seeds of the plant showed significant activity against Candida krusei insert ignore into journalissuearticles values(MIC = 62.5 µg/mL);. To the best of our knowledge, the current work is the first to report the alkaloid profile and antimicrobial activity of S. alopecuroides var. alopecuroides growing in Turkey.
Keywords : Key words Sophora, Sophora alopecuroides var alopecuroides, Leguminosae, alkaloid, antimicrobial activity, GC MS