Biological activities of Cu(II) and Hg(II) complexes of a heptadentate Schiff base ligand

Authors : Emel ERGENE, Hülya SİVAS, Kadriye BENKLİ

Pages : 379-387

View : 12 | Download : 5

Publication Date : 2010-12-01

Article Type : Research Paper

Abstract :Two metal complexes were synthesized as [Hginsert ignore into journalissuearticles values(L);]insert ignore into journalissuearticles values(ClO4);2 and [Cuinsert ignore into journalissuearticles values(L);]insert ignore into journalissuearticles values(ClO4);2 by the template reaction between 2,6-bisinsert ignore into journalissuearticles values(2 aminothiophenoxymethyl);pyridine and 2,2`-bipyridine-6,6`-dicarboxaldehyde in the presence of Hginsert ignore into journalissuearticles values(II); and Cuinsert ignore into journalissuearticles values(II); perchlorate salts. The structures of the compounds were elucidated by IR, 1H-NMR, MASS, and Elemental Analyses values. Cytotoxicity of the compounds were investigated by MTT [3-insert ignore into journalissuearticles values(4,5-dimethylthiazol-2-yl);-2,5-diphenyltetrazolium bromide] assay in normal and cancerous rat fibroblasts. Both compounds showed cytotoxicity on 2 cell lines as a dose-dependent manner. Compound [Hginsert ignore into journalissuearticles values(L);]insert ignore into journalissuearticles values(ClO4);2 was more cytotoxic than [Cuinsert ignore into journalissuearticles values(L);]insert ignore into journalissuearticles values(ClO4);2. Apoptotic activity of compounds was evaluated by acridine orange staining and DNA fragmentation assay. Both cell lines exposed to the compounds exhibited condensed chromatin and appearance of apoptotic bodies. The percentages of all these abnormalities were found to be very high level in ras-transformed 5RP7 fibroblasts. The effect of [Hginsert ignore into journalissuearticles values(L);]insert ignore into journalissuearticles values(ClO4);2 was again more stronger than the compound [Cuinsert ignore into journalissuearticles values(L);]insert ignore into journalissuearticles values(ClO4);2. Although the compound [Cuinsert ignore into journalissuearticles values(L);]insert ignore into journalissuearticles values(ClO4);2 induced the formation of DNA fragmentation in both cell lines, [Hginsert ignore into journalissuearticles values(L);]insert ignore into journalissuearticles values(ClO4);2 induced the DNA fragmentation only in cancerous 5RP7 cells, indicating specific activity. In conclusion, we suggest that both complexes, especially the one with Hginsert ignore into journalissuearticles values(II); according to its significant differences of apoptotic morphology and DNA fragmentation, exhibit promising potentials as anticancer compounds.
Keywords : Key words Metal complexes, apoptosis, DNA fragmentation, cancer cells, MTT