Ultrastructural changes in rat liver by methyleugenol and evaluation of some biochemical parameters

Authors : Ayşegül ÇERKEZKAYABEKİR, Gülnur KIZILAY, Figen ERTAN

Pages : 439-445

View : 12 | Download : 3

Publication Date : 2010-12-01

Article Type : Research Paper

Abstract :The present study investigated the effect of methyleugenol, a food flavoring and fragrance agent, on the livers of laboratory rats. Doses of 10 and 30 mg/kg/day of methyleugenol insert ignore into journalissuearticles values(in 0.5% methylcellulose); were administered intragastrically insert ignore into journalissuearticles values(IG); to 2 dose groups for 10 days alongside a control group insert ignore into journalissuearticles values(n = 10 for each group);. Gains in body weight were not statistically significant for either dose. Lactate dehydrogenase insert ignore into journalissuearticles values(LDH); and alkaline phosphatase insert ignore into journalissuearticles values(ALP); activities decreased insert ignore into journalissuearticles values(P < 0.05); for both dose groups, but AST insert ignore into journalissuearticles values(aspartate aminotransferase); activity decreased significantly insert ignore into journalissuearticles values(P < 0.05); only in the 30 mg/kg/day methyleugenol dose group. Alanine aminotransferase insert ignore into journalissuearticles values(ALT); activity did not change significantly in either group. The total amount of glucose remained unchanged, but glycogen in the liver decreased significantly insert ignore into journalissuearticles values(P < 0.05); in the 30 mg/kg/day methyleugenol dose group. There was also an increase of swelling in the smooth endoplasmic reticulum sacs insert ignore into journalissuearticles values(sER);, electron dense accumulations in the sER, cytoplasmic vacuolization, a slight increase of mitochondria and lysosomes, and invaginations in the nucleus membranes of hepatocytes. Extensions in bile canaliculi and increasing microvilli of bile canaliculi in the liver were also observed. These findings indicate the ultrastructural toxic effect of methyleugenol on rat livers in 10 and 30 mg/kg/day doses. Therefore, we may speculate that the toxic effect of methyleugenol needs to be examined in detail.
Keywords : Key words Methyleugenol, liver, ultrastructure, ALT, AST, LDH, ASP, glucose, glycogen, rat