Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

Authors : Sevil ALBAYRAK, Ahmet AKSOY, Osman SAĞDIÇ, Ümit BUDAK

Pages : 463-473

View : 11 | Download : 5

Publication Date : 2010-12-01

Article Type : Research Paper

Abstract :The antioxidant activity of the methanolic extracts of 4 plants from genus Helichrysum Mill. insert ignore into journalissuearticles values(Asteraceae); [H. arenarium insert ignore into journalissuearticles values(L.); Moench subsp. erzincanicum Davis & Kupicha, H. arenarium insert ignore into journalissuearticles values(L.); Moench subsp. rubicundum insert ignore into journalissuearticles values(C.Koch.); Davis & Kupicha, H. armenium DC. subsp. araxinum insert ignore into journalissuearticles values(Kirp.); Takht., and H. plicatum DC. subsp. pseudoplicatum insert ignore into journalissuearticles values(Nab.); Davis & Kupicha] was investigated with 2 complementary test systems, DPPH insert ignore into journalissuearticles values(2,2-diphenyl-1-picrylhydrazyl); radical scavenging and phosphomolybdenum assay. H. plicatum subsp. pseudoplicatum showed the highest level of total antioxidant activity insert ignore into journalissuearticles values(161.79 ± 0.3 mg ascorbic acid equivalent/g extract); in the phosphomolybdenum assay. H. arenarium subsp. erzincanicum showed the highest level of free radical scavenging activity, with an IC50 value of 23.03 µg/mL. The total phenolic contents of the extracts ranged from 71.81 to 144.50 mg gallic acid/g dry extract. Chlorogenic acid, apigenin-7-glucoside, and apigenin were found by HPLC analysis to be the main components in the extracts. Antimicrobial activity was investigated with the agar diffusion method. The methanolic extracts had antibacterial activity against Aeromonas hydrophila, Bacillus brevis, B. cereus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC 29213. However, no activity was found against Escherichia coli, Morganella morganii, Mycobacterium smegmatis, Proteus mirabilis, Yersinia enterocolitica, or Saccharomyces cerevisiae.
Keywords : Key words Helichrysum, antimicrobial activity, antioxidant activity, phenolic compounds, DPPH