The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips

Authors : Sanjay KUMAR, - *, Shashi Kiran ARYA, Bijoy Krishna ROY, Atul Kumar SINGH

Pages : 55-66

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Publication Date : 2010-12-01

Article Type : Research Paper

Abstract :The effects of the herbicides 2,4-dichlorophenoxy acetic acid and isoproturon on 3 wheat insert ignore into journalissuearticles values(Triticum aestivum L.); varieties insert ignore into journalissuearticles values(HUW 234, HUW 468, and HUW 533); were studied with regards to mitotic abnormalities and chromosomal behavior. Pre-soaked seeds were treated with both herbicides at concentrations of 50-1200 ppm. Both 2,4-D and isoproturon were highly mito-inhibitory and induced chromosomal abnormalities, such as precocious movement, stickiness, and chromosome bridges, with and without laggards and fragments at the anaphase and telophase. The frequency of chromosomal anomalies in almost all the targets used was high at the maximum dose of both herbicides, individually as well as in combination. Isoproturon was more toxic, as it resulted in high-level chromosomal damage in all the varieties. Both herbicides had a dose-dependent impact on the mitotic index insert ignore into journalissuearticles values(MI); and relative abnormality rate insert ignore into journalissuearticles values(RAR);. These parameters collectively indicate that variety HUW 468 was more susceptible to the mito-depressive and chromotoxic action of the 2 herbicides.
Keywords : Key words Isoproturon, 2, 4 dichlorophenoxy acetic acid, Tritium aestivum L, chromosomal abnormalities, mitosis, relative division rate RDR, , relative abnormality rate insert ignore into journalissuearticle