Characterization of Rhizobium Sp. Isolated from Bean

Authors : Çiğdem KÜÇÜK, Merih KIVANÇ, Engin KINACI

Pages : 127-132

View : 15 | Download : 5

Publication Date : 2006-12-01

Article Type : Research Paper

Abstract :Thirty nodule isolates from bean insert ignore into journalissuearticles values(Phaseolus vulgaris L.); grown in Eskişehir were studied for their physiological and biochemical characteristics. Most isolates produced abundant extracellular polysaccharides, tolerated high salt concentration insert ignore into journalissuearticles values(5% NaCl);, grew at a temperature of 42 °C, and synthesized melanin. They were able to grow at pHs ranging from 3.5 to 9.0. The majority of the isolates showed an intrinsic resistance to the antibiotics chloramphenicol insert ignore into journalissuearticles values(20 and 50 µg ml-1);, erythromycin insert ignore into journalissuearticles values(30 µg ml-1);, kanamycin insert ignore into journalissuearticles values(10 µg ml-1);, and streptomycin insert ignore into journalissuearticles values(40, 80, and 100 µg ml-1);. Plasmid DNA profiles of the isolates were identified and it was determined that 2 isolates contained plasmid DNA from 1 to 3.
Keywords : Rhizobium sp, bean Phaseolus vulgaris L, , isolation, isolate