Biological Activities of the Essential Oil and Methanol Extract of Achillea biebersteinii Afan. (Asteraceae)

Authors : Özlem BARIŞ, Medine GÜLLÜCE, Fikrettin ŞAHİN, Hakan ÖZER, Hamdullah KILIÇ, Hakan ÖZKAN

Pages : 65-73

View : 15 | Download : 3

Publication Date : 2006-12-01

Article Type : Research Paper

Abstract :The present work examined the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Achillea biebersteinii Afan. insert ignore into journalissuearticles values(Asteraceae);. The essential oil exhibited antimicrobial activity against 8 bacteria, 14 fungi and the yeast C. albicans, whereas methanolic extract remained inactive. The antioxidative capacity of the samples was evaluated by using DPPH insert ignore into journalissuearticles values(2,2-diphenyl-1-picrylhydrazyl); and b-carotene/linoleic acid assays. In both assays, the extract showed better antioxidative capacity than the oil. The extract reduced the stable free radical DPPH with lower IC50 value insert ignore into journalissuearticles values(89.90 µg/ml); than the oil insert ignore into journalissuearticles values(8900 µg/ml);. In the b-carotene/linoleic acid assay, the samples were not effectively able to inhibit the linoleic acid oxidation, exhibiting only 22.7% insert ignore into journalissuearticles values(the extract); and 16% insert ignore into journalissuearticles values(the essential oil); inhibitions at 2 mg/ml, far below than that of BHT insert ignore into journalissuearticles values(97.0%);. Total phenolic constituent of the extract was 51 µg/mg insert ignore into journalissuearticles values(5.1%, w/w); as gallic acid equivalent. GC-MS analysis of the essential oil resulted in the identification of 64 components representing 92.24% of the oil. Piperitone, camphor and 1,8-cineole insert ignore into journalissuearticles values(eucalyptol); were the main constituents.
Keywords : Achillea biebersteinii, Antimicrobial activity, Antioxidant activity, GC MS analysis, piperitone, camphor, eucalyptol