Effects of Carbon Sources and Various Chemicals on the Production of a Novel Amylase from a Thermophilic Bacillus sp. K-12

Authors : Özlem KIRAN, Uğur ÇÖMLEKÇİOĞLU, Burhan ARIKAN

Pages : 99-103

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Publication Date : 2005-12-01

Article Type : Research Paper

Abstract :The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmaraş. Enzyme synthesis occurred at 20-55 ºC with an optimum of 42 ºC. There was a slight variation in amylase synthesis within the pH range 4.5-10.5. Effects of various carbon sources and chemicals on a-amylase production were examined and maximum a-amylase production was obtained in a medium containing 1% starch in 60 h. MnSO4, ZnSO4 and EDTA inhibited a-amylase production of Bacillus sp. K-12.
Keywords : Thermostable, Amylase, Bacillus