First Record of a NPV Isolated from Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) in Turkey and Its Molecular Identification According to the Partial lef-8 Gene

Authors : Umut TOPRAK

Pages : 71-77

View : 15 | Download : 3

Publication Date : 2004-12-01

Article Type : Research Paper

Abstract :Spodoptera littoralis insert ignore into journalissuearticles values(Boisd.); insert ignore into journalissuearticles values(Lepidoptera: Noctuidae); is an important pest in Turkey and causes extensive economic losses in many cultivated crops. The viruses within the family Baculoviridae are specific pathogens of insects. The nucleopolyhedroviruses insert ignore into journalissuearticles values(NPVs);, 1 of the 2 genera of this family, have been isolated from many insect orders, primarily from the lepidopterans. In our study, a local NPV was isolated from the larvae of Spodoptera littoralis fed in the cotton fields in southern Turkey insert ignore into journalissuearticles values(Mersin);. For the molecular identification of the virus, polyhedra insert ignore into journalissuearticles values(occlusion bodies [OBs]); were isolated from insects and total DNA extraction was performed using a commercial kit from OBs. One of the genes involved in late gene expression found in the SpliNPV genome, lef-8, was partially amplified by PCR. Nucleotide sequence analysis of this region was performed by MWG insert ignore into journalissuearticles values(Ebersberg, Germany);. According to the nucleotide sequences of partial lef-8, searches carried out with the updated GenBanks showed that the isolate was a Spodoptera littoralis NPV-B variant. This NPV was the fifth SpliNPV-B variant isolated worldwide and was named SpliNPV-TR1 isolate as it is the first record in Turkey of a NPV isolated from Spodoptera littoralis.
Keywords : Baculovirus, NPV, Spodoptera littoralis, lef 8, PCR, Nucleotide sequence analysis