Partial Purification of Intestinal Triglyceride Lipase from Cyprinion macrostomus Heckel, 1843 and Effect of pH on Enzyme Activity

Authors : Naci DEĞERLİ

Pages : 133-143

View : 11 | Download : 3

Publication Date : 2002-12-01

Article Type : Research Paper

Abstract :Intestinal triglyceride lipase insert ignore into journalissuearticles values(TG); of Cyprinion macrostomus after precipitation with 30% polyethylene glycol insert ignore into journalissuearticles values(PEG);, was partially purified by hydrophobic interaction chromatography on phenyl sepharose CL-4B, and the effect of pH on this enzyme activity was determined titrimetrically. Intestinal tissue homogenates were precipitated using 30% PEG-8000, and then applied onto a phenyl sepharose CL-4B column for hy-drophobic interaction chromatography. The lipase that bound to this hydrophobic resin and was partially purified by this single step application was then eluted from this resin with taurocholic acid and Triton X-100 elution. This purified enzyme effectively hydrolysed natural olive oil substrate under the experimental conditions. An optimal pH of 7.50, and 94.37% yield, and 71.54-fold purification parameters were estimated for this lipase. The molecular mass of this enzyme was determined to be 51 kDa under non-denaturing conditions by polyacrylamide disc-gel electrophoresis insert ignore into journalissuearticles values(Disc-PAGE);. It was concluded that the intestinal lipase of this fish is alkaline in character.
Keywords : Lipase, purification, enzyme activity, optimal pH