Protoplast Fusion in Sugar Beet (Beta vulgaris L.)

Authors : Songül GÜREL

Pages : 163-170

View : 10 | Download : 5

Publication Date : 2002-12-01

Article Type : Research Paper

Abstract :In this study, attempts were made to fuse the suspension-derived protoplasts of a sugar beet breeding line insert ignore into journalissuearticles values(ÇBM315); with the mesophyll-derived protoplasts of three breeding lines insert ignore into journalissuearticles values(M114, ELK345 and M1017);, which were obtained from our previous experiments. Combinations of three different PEG concentrations insert ignore into journalissuearticles values(20%, 25% or 30%); and three different treatment durations insert ignore into journalissuearticles values(15, 20 or 25 min); were tested. The fusion efficiency was determined by measuring the viability, heterokaryon frequency and plating efficiency insert ignore into journalissuearticles values(PE); of the fused protoplasts after four weeks of culture. In general, 25% PEG combined with 20 min treatment duration produced the highest fusion frequency, but the highest rate of viability was obtained at 20% PEG with 25 min treatment duration. The capacity of heterokaryons to form cell colonies was highest when lower PEG insert ignore into journalissuearticles values(20 or 25%); was used for shorter treatment durations insert ignore into journalissuearticles values(15 or 20 min);. No macrocallus or regeneration from the fused protoplasts was achieved.
Keywords : Sugar beet, protoplast fusion, PEG, breeding lines