Purification and Determination of the Molecular Structure of Hemolymph Lectin of Agrotis segetum (Denis and Schiff.)

Authors : Nursel GÜL, Cevat AYVALI

Pages : 49-55

View : 11 | Download : 3

Publication Date : 2002-12-01

Article Type : Research Paper

Abstract :A soluble lectin was purified from the hemolymph of Agrotis segetum by two methods, via affinity chromatography on a Sepharose-4B column and gel filtration on a Superdex-200 column. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis insert ignore into journalissuearticles values(SDS-PAGE); showed a single protein band with a molecular weight of 69,000 in both the presence and absence of 2-Mercaptoethanol. Alternatively, the molecular weight of this lectin was estimated to be 69,000 by gel filtration on Superdex-200. Electron microscopic observation of the purified lectin was reticular in structure and consisted of tandem aligned spherical basic units.
Keywords : Agrotis segetum, lectin agglutinin, , purification, molecular weight, transmission electron microscopy TEM,