Purification of Buchnericin LB Produced by Lactobacillus buchneri LB

Authors : Purification Of Buchnericin Lb Produced BY

Pages : 59-65

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Publication Date : 2001-12-01

Article Type : Research Paper

Abstract :Buchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a rapid and simple three-step purification procedure including freeze drying, silicic acid adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity increased about 111 and 2,500 fold, respectively. It was determined that the adsorption of buchnericin LB to silicic acid and cation-exchange chromatography was dependent on the pH of the suspending environment. The molecular weight of buchnericin LB was determined to be about 4.0 kDa by tricine SDS-PAGE.
Keywords : Bacteriocin, buchnericin LB, Lactobacillus bunchneri LB, purification, silicic acid, cation exchange chromatography