Production of Monoclonal Antibodies Specific for Progesterone, Estradiole by Simultaneous Injection of Different Steroids

Authors : Fatima Şahingöz YÜCEL

Pages : 697-706

View : 11 | Download : 3

Publication Date : 2000-12-01

Article Type : Research Paper

Abstract :We report here the development of hybrid cells producing monoclonal antibodies specific for two different steroid hormones with mixed immunization using hybridoma technology. BALB/c mice were immunized with a mixture of three steroid antigens: progesterone, estradiole and testosterone linked to bovine serum albumine. These mice were used for fusion. In the two fusion experiments, ELISA tests showed that among 645 wells only 2 hybrids reacted with progesterone insert ignore into journalissuearticles values(MAM 3C2, MAM 3E3); and one of them reacted specifically with estradiol insert ignore into journalissuearticles values(MAM 7H7);. Three types of monoclonal antibodies were detected: IgG1, IgG2a and IgG2b and the affinities of these antibodies were found to be 10 -8 -10 -9 M. Monoclonal antibodies did not cross react with the other steroid hormones.
Keywords : Immunodiagnosis, hybrid cell, monoclonal antibody, steroid hormones, ELISA